|
K562 cells were the first human immortalised myelogenous leukemia line to be established. K562 cells are of the erythroleukemia type, and the line is derived from a 53-year-old female CML patient in blast crisis.〔 〕〔 〕 The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes〔 〕 and erythrocytes.〔 〕 In culture they exhibit much less clumping than many other suspension lines, presumably due to the downregulation of surface adhesion molecules by bcr:abl. However, another study suggests that bcr:abl over-expression may actually increase cell adherence to cell culture plastic. K562 cells can spontaneously develop characteristics similar to early-stage erythrocytes, granulocytes and monocytes〔 〕 and are easily killed by natural killer cells〔 〕 as they lack the MHC complex required to inhibit NK activity.〔 They also lack any trace of Epstein-Barr virus and other herpesviruses. In addition to the Philadelphia chromosome they also exhibit a second reciprocal translocation between the long arm of chromosome 15 with chromosome 17.〔 Two sub-lines are available which express MHC class-I A2〔 〕 and A3.〔 〕 ==References== 〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「K562 cells」の詳細全文を読む スポンサード リンク
|